Abdel Karim. M. , Asma,H., and Kamal, M.S.

DOI:

VOLUME-5, ISSUE-12

GC-MS Analysis and Antimicrobial Activity of Sudanese Cucumis melo L. (Cucrbitaceae ) Fixed Oil

Abdel Karim. M. , Asma,H., and Kamal, M.S.

1. Sudan University of Science and Technology, Faculty of Science, Dept. of Chemistry

2. University of El-Fashir , Faculty of Education, Dept. of Chemistry

ABSTRACT

The present study was designed to investigate the chemical constituents of Sudanese Cucumis melo seed oil and to evaluate its potential antimicrobial activity. 28 components were detected by GC-MS analysis. Major constituents are: methyl 10-trans,12-cis-octadecadienoate (49.28%) , 9-octadecenoic acid methyl ester(19.40%) , hexadecanoic acid methyl ester(15.92%) , methyl stearate(11.22%) The antimicrobial activity of the oil was evaluated via cup plate agar diffusion bioassay against six standard human pathogens(Gram positive: Staphylococcus aureus and Bacillus subtilis; Gram negative : Escherichia coli and Pseudomonasa aeruginosa and the fungi Candida albicans and Aspergillus niger) . The oil showed different antimicrobial responses against test organisms. It gave significant activity against the fungus :Candida albicans and partial activity against Staphylococcus aureus.It seems that the oil is a lead for further optimization.

Keywords: Cucumis melo, Fixed oil, GC-MS, Antimicrobial activity

Full Text Article