PRELIMINARY PHYTOCHEMICAL SCREENING AND PROTECTIVE EFFECT OF ERYTHRINA SENEGALENSIS IN AN IN VIVO MOUSE MODEL OF ALZHEIMER S DISEASE

Marcellin Koffi Ahue,1 Janat Akhanovna Mamyrbekova-Bekro,1 Tangui Maurice,2,3,4 David Virieux,5 Jean-Luc Pirat,5 Yves-Alain Bekro1*

1Laboratoire de Chimie Bio-Organique et de Substances Naturelles (LCBOSN/ www.lablcbosn.com), UFR-SFA, Université Nangui Abrogoua, 02 BP 801 Abidjan 02, Côte d Ivoire

ABSTRACT

Erythrina senegalensis is a therapeutic plant of the African Pharmacopoeia, widely used by populations in the treatment many pathologies, including mental disorders and cognitive impairments. We qualitatively analyzed crude methanol, acidic extracts and selective extracts from bark of E. senegalensis by identification tests, and evaluated its anti-radical activity (DPPH test) and compared to vitamin C. Finally, we examined its neuroprotective potential in vivo in a pharmacological mouse model of 631 Journal of Medical Pharmaceutical and Allied Sciences (March_2017); 630-644 Alzheimer s disease at the behavioral and biochemical level. The neuroprotective activity was evaluated in vivo in mice intracerebroventricularly injected with oligomeric amyloid-β25-35 peptide. The different tests were analyzed and compared to donepezil, an acetylcholinesterase inhibitor. Sterols, terpenes, coumarins, alkaloids, flavonoids, polyphenols and tannins were identified in the plant. The best inhibitory profile of DPPH radical was observed for the chloroform extract. The crude methanol extract had high neuroprotective activities in vivo.

Keywords: Erythrina senegalensis, extract, DPPH, neuropro-tective activity, Alzheimer s disease.