Multivariate calibration technique for spectrophotometric quantification of ondansetron in bulk drug and pharmaceutical formulations

Kokilambigai K S, Logesh M, Anusha Reddy C, Seetharaman R, Kavitha J, Lakshmi K S

Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India

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Logesh M, Kokilambigai K S, Anusha Reddy C, Seetharaman R, Kavitha J, Lakshmi K S, 2022. Multivariate calibration technique for spectrophotometric quantification of ondansetron in bulk drug and pharmaceutical formulations. Journal of medical pharmaceutical and allied science. V 11 – I 4, Pages - 5050 - 5056 doi: 10.55522/jmpas.V11I4.2550.

ABSTRACT

The aim of the present work to develop an accurate, simple, sensitive, and validated Ultra violet (UV) spectrophotometric procedure using multilinear regression method for the analysis of Ondansetron in its bulk and commercial dosage form. The multivariate calibration approach was based on equations constructed using linear regression analysis using the correlation between absorbance and concentration at selected five different wavelengths. Ondansetron had a maximum wavelength of 246 nm. The findings were statistically analysed. A linear plot in the concentration range of 5-15 μg/mL, with a regression co-efficient of 0.999 was obtained. The % RSD for intra-day and Inter day precision were 0.0409 and 0.0228, respectively. The assay was determined and found to be 98.38 % – 101.10 % w/w.

Keywords:

Ondansetron, Antiemetic agent, UV spectrophotometer, Multivariate calibration, ICH guidelines

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