Anti-migration, anti-proliferative, and pro-apoptotic effects on cancer cell line HCT116 using sponge extracts from Lugait, Misamis Oriental, Philippines

Toledo, Nenito, Lavilla, Charlie Jr., Uy, Mylene

Department of Chemistry, College of Science and Mathematics, Mindanao State University – Iligan Institute of Technology, Iligan City, Philippines.

Refer this article

Toledo, Nenito, Lavilla, Charlie Jr., Uy, Mylene, 2022. Anti-migration, anti-proliferative, and pro-apoptotic effects on cancer cell line HCT116 using sponge extracts from Lugait, Misamis Oriental, Philippines. Journal of medical pharmaceutical and allied sciences, V 11 - I 6, Pages - 5406 – 5414. Doi: 10.55522/jmpas.V11I6.4368.

ABSTRACT

Sponge samples coded KL-03P, KL-12P, KL-15P, KL-16P, and KL-18NP collected from Lugait, Misamis Oriental, Philippines were subjected to fractionation resulting in hexane (H), dichloromethane (D), and aqueous (A) fractions of each sponge. They were subsequently subjected to anti-migration, anti-proliferative, and pro-apoptotic activity testing using the Scratch Wound assay, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, and the Caspase Glo 3/7 assay, respectively. Fractions KL-03P (D), KL-12P (D), KL-16P H) and KL-18NP (D) showed anti-proliferative and pro-apoptotic effects against HCT116 at 30-ppm concentration. Cell shrinkage and noticeable fragmentations indicative of apoptosis were apparent among these fractions based on MTT final snaps. But among these active fractions, the unprecedented abilities of KL-18NP (D) were very notable across assays conducted. This fraction showcased the inhibition of cell migration, which gave a signal for potentially reduced oncogenicity or metastatic capability of HCT116. The negative percentage of wound healing exhibited by this fraction is a manifestation of its cytotoxicity effect. Very remarkably, KL-18NP (D) has a similar degree of responses across the positive controls, respectively. Hence, there is a significant negative relationship between the negative control versus KL-18NP (D) in terms of its anti-migratory and anti-proliferative properties, p<0.05. Additionally, there is a significant positive relationship between the negative control and KL-18NP (D) when it comes to its pro-apoptotic capability, p<0.05. These results make KL-18NP (D) a bioactive hit as an anti-cancer agent source. Confirmatory techniques, structure determination, and target identification methods will further ascertain its mechanism of action as well as the identification of its cellular targets.

Keywords:

Anti-Migration; Anti-Proliferative; Pro-Apoptotic; Fold Change; Tiering Classification.

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