Combination treatment of Chromolaena odorata ethanolic extract with cisplatin against breast cancer cell lines MDA-MB-231 and MCF-7

Nurliana ABD Mutalib, Zulhilmi Mohamad Bakri, Khairuddin Abdul Jalil, Normala ABD Latip

Faculty of Pharmacy, Universiti Teknologi MARA Cawangan Selangor,Puncak Alam, Selangor, Malaysia

Refer this article

Normala ABD Latip, Nurliana ABD Mutalib, Zulhilmi Mohamad Bakri, Khairuddin Abdul Jalil, 2023. Combination treatment of Chromolaena odorata ethanolic extract with cisplatin against breast cancer cell lines MDA-MB-231 and MCF-7. Journal of medical pharmaceutical and allied sciences, V 12 - I 2, Pages – 5740 – 5744. Doi: 10.55522/jmpas. V12I2.4028.

ABSTRACT

Many cancer patients are using complementary and alternative medicines (CAM) in combination with their conventional therapy, more than 72% of them do not inform their physician about CAM use. With the use of CAM in combination with conventional chemotherapeutics, there is an increasing risk for unwanted interactions. Chromolaena odorata locally known as ‘pokok kapal terbang’ and traditionally used for wound healing. However, several studies have been done on cytotoxicity of C. odorata against various type of cell lines. Thus, in this study, interaction between cisplatin and C. odorata treatment on breast cancer cell lines was evaluated. C. odorata leaves were extracted using maceration method with 70% ethanol and the extraction yield was 2.69% (w/w). MCF-7 and MDA-MB-231 cell lines were treated with C. odorata ethanolic extract in combination with cisplatin using MTT assay. Combination index (CI) was calculated and an isobologram was constructed to evaluate interactions between plant extract and drug. The combination of cisplatin ethanolic extract of C. odorata resulted in antagonism with a combination index greater than one.

Keywords:

Chromolaena odorata, MCF-7, MDA-MB-231, MTT assay, Cisplatin, Breast cancer, Combination index, Iso-bologram.

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