Phytochemical profile and bioactive compounds of beluntas leaves extract (PLUCHEA INDICA L.) and its hydrogel preparations

Nur Habibah, Heru Santoso Wahito Nugroho, I Gusti Ayu Sri Dhyanaputri, I Gusti Agung
Ayu Dharmawati, Ida Bagus Oka Suyasa

Department of Health, Poltekkes Kemenkes Surabaya, Indonesia.

Refer this article

Nur Habibah, Heru Santoso Wahito Nugroho, I Gusti Ayu Sri Dhyanaputri, I Gusti Agung Ayu Dharmawati, Ida Bagus Oka Suyasa, 2023. Phytochemical profile and bioactive compounds of beluntas leaves extract (PLUCHEA INDICA L.) and its hydrogel preparations. Journal of medical pharmaceutical and allied sciences, V 12 - I 6, Pages - 6184 – 6190. Doi: https://doi.org/10.55522/jmpas.V12I6.5672.

ABSTRACT

“Beluntas” (Pluchea indica L.) is a plant that has been used as a traditional medicinal plant for various types of diseases. The most common formulation for Pluchea indica L. utilization is in the form of extract. However, Pluchea indica L. extract cannot be used directly. It is needed to develop in other formulations, such as a hydrogel. This research aimed to determine the phytochemical profile, the content of bioactive compounds, and the preparation of Pluchea indica L. extract incorporated carbopol-HPMC hydrogel. This type of research was a quasi-experimental method. The sample in this study was Pluchea indica L. extract. Qualitative tests were carried out for the determination of the phytochemical profile. At the same time, the content of total phenol, flavonoids, total alkaloids, tannins, and antioxidant activity was analyzed using the Folin-Ciocalteu, AlCl3, Folin-Denis, and DPPH methods by visible spectrophotometry. The results showed that Pluchea indica L. extract had several phytochemical compounds, namely: alkaloids, tannins, flavonoids, and phenols. The highest level of bioactive compounds was found at a parameter of total phenol with a value of 456.431 mg/g. The sample had strong antioxidant activity, expressed with an IC50 value of 0.503 ppm. Preparation of Pluchea indica L. extract incorporated carbopol-HPMC hydrogel produced hydrogel that meets the consistency, color, scent, homogeneity, spreadability, pH, and viscosity requirements.

Keywords:

Beluntas” leaves extract, Phytochemical profile, Bioactive compounds, Hydrogel preparation.

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