Bio-analytical study of Efonidipine Hydrochloride Ethanolate and Telmisartan in human plasma by RP-HPLC

Vaishnavi Nathe, Jagdish Bharsawde, Pranali Polshettiwar, Amol More, Ganesh Choudhari*

School of Health Sciences and Technology, Dr. Vishwanath Karad, MIT World Peace University, Pune, Maharashtra, India

Refer this article

Vaishnavi Nathe, Jagdish Bharsawde, Pranali Polshettiwar, Amol More, Ganesh Choudhari, 2025. Bio-analytical study of efonidipine hydrochloride ethanolate and telmisartan in human plasma by RP-HPLC. Journal of medical pharmaceutical and allied sciences, V 14 - I 3, Pages - 16 – 23. Doi: https://doi.org/10.55522/jmpas.V14I3.6858.

ABSTRACT

Hypertension is now commonly treated with two or three-drug combinations, often using two drugs. So, a binary drug combination was selected for this analytical study. The formulation comprises Efonidipine HCl Ethanolate (EFN) and Telmisartan (TEL), which treat cardiovascular conditions such as hypertension. Amlodipine was used as the Internal Standard (IS) to develop and validate a new and reliable RP-HPLC analytical technique for the simultaneous analysis of EFN and TEL in human plasma. Method novelty, sensitivity, rapidity, precision, and accuracy were evaluated.

Keywords:

This process involved spiking 50?L of EFN, 50?L of TEL, and 50?L of Amlodipine (IS) to a 350?L sample of human plasma, which was then extracted using solid phase extraction (SPE). Utilizing an Agilent RP-HPLC system featuring a Shimadzu column C18 (250

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