A novel application of hydrotropic solubilization for quantitative estimation and validation of Atenolol and Hydrochlorothiazide in tablet dosage form

Rathore Pooja, Bainerjee Bhasker, Bainerjee Tanushree, Jain Prabhat, Bajpai Dinesh

R K D F College of Pharmacy, Hoshangabad Road, Bhopal, India

ABSTRACT

Simple, sensitive, economical analytical methods are described for the determination of Atenolol and hydrochlorothiazide in bulk and tablet formulations. In the present investigation, 4M Ammonium acetate solution (Hydrotropic agent) was employed to solubilizing, Atenolol and hydrochlorothiazide (a poorly water soluble drug), from fine powder of its tablets to carryout spectrophotometric analysis. The proposed methods namely simultaneous equation method (Method 1) and absorbance ratio method (Method-2). λmax for Atenolol and hydrochlorothiazide is 224.5 nm and 272.5 nm respectively. Both Atenolol and hydrochlorothiazide obey Beer’s law in the concentration range of 10-50 μg/ml (r2=0.9992) and 5-25 μg/ml (r2=0.9994) in 4 M Ammonium acetate (Hydrotropic agent) respectively. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values.

Keywords: Hydrotropic solubilization, Spectrophotometry methods, ammonium acetate, ICH guideline.