Development and Validation of Spectrophotometric Method with Hydrotropic Solubilizing Agent for Simultaneous Estimation of Domeperidone and Paracetamol in Solid Dosage Form

Yadav S.K.¹, Tiwari Vibhanshu*¹, Pathak Aashish¹, Jain Prabhat¹, Bajpai Dinesh²

Ravishanker College of pharmacy, Karod, Bhopal

ABSTRACT

A simple, precise and UV-Visible spectroscopic method was developed and validated for simultaneous estimation of domeperidone an anti emetic and paracetamol an NSAID’S in solid dosage form” The identification of both drugs was achieved on 8 M Urea solution as a suitable solvent in all temperature , Absorbance of the sample solutions at 287.0 nm and 248.0 nm was measured and from the appropriate absorbance values, Both domeperidone and paracetamol obey Beer’s law in the concentration range of 10-50 μg/ml. the concentration of drugs in the sample solution was determined by using Vierodt's formula. The method was successfully validated in accordance to ICH guidelines. Values of accuracy, precision and other statistical analysis were found to be in good according with the standard values. Further, the validated method was applied for commercially available pharmaceutical dosage form.

Keywords: Validation, simultaneous, solubilization, ICH guideline, spectroscopy