MOLECULAR CHARACTERIZATION OF RHIZOBIUM OF CAJANUS CAJAN PLANT FROM SALT AND HERBICIDAL ACTION

Shende R.C. & Patil M.B.

Department of Biochemistry, L.I.T premises. Nagpur University, Amravati Road, Nagpur-440033.

ABSTRACT

The genetic diversity among the in vitro and in vivo Rhizobium species assessed by RAPD-DNA finger printing and PCR was done. Variations between the Rhizobium species of cajanus cajan plant; normal plant grown in normal conditions (N), normal plant in presence of 4% ai glyphosate (Ng), normal plant in presence of 300mM NaCl i.e Hallophillic (H) and normal plant in presence of 300mM NaCl and 6% ai glyphosate ie Hallophillic glyphosate (Hg) was concluded. Salinity-induced changes in the DNA profiles in Rhizobium sp. it exhibited alterations in the expression of Hg, which either showed an enhanced rate of synthesis as compared with their respective controls. RAPD tests categorized the isolates into two clusters. The RAPD results were further analyzed by MVSP software; similarity matrix was measured and converted into dendrogram using UPGMA clustering method.

Keywords: Cajanus Cajan, DNA, RAPD-PCR, Rhizobium, Nodules and stress.