DOI: 10.55522/jmpas.v2i4.0022
AUGUST 2013
RC Shende*, MB Patil
Department of Biochemistry, L.I.T premises. Nagpur University, Nagpur, Maharashtra, India
Refer this article
RC Shende, MB Patil, 2013. Effects of glyphosate on metabolism of phenolic compounds (pal). Journal of medical pharmaceutical and allied sciences, V 2 - I 4, Pages -82 – 85. Doi: https://doi.org/10.55522/jmpas.v2i4.0022.
ABSTRACT
The present study had as a goal to purify and characterize the enzyme Phenyl alanine ammonia lyase from the nodules and Rhizobia of cajanus cajan plant grown in NaCl and glyphosate conditions. N (grown in normal condition), Ng (grown in 4% ai glyphosate), H (grown in 300mM NaCl) & Hg (grown in 300mM and 6%ai glyphosate). In vitro and in vivo studies of enzyme PAL, isolation, purification, substrate specificity, optimum pH and temperature, activators and inhibitors, kinetic properties, molecular weight by SDS-PAGE and the action of glyphosate on PAL enzyme was checked.
Keywords:
Phenyl alanine ammonia lyase, Cajanus cajan, Rhizobium, SDS-PAGE, Glyphosate.