EFFECTS OF GLYPHOSATE ON METABOLISM OF PHENOLIC COMPOUNDS (PAL)

Shende R.C. & Patil M.B.

ABSTRACT

The present study had as a goal to purify and characterize the enzyme Phenyl alanine ammonia lyase from the nodules and Rhizobia of cajanus cajan plant grown in NaCl and glyphosate conditions. N (grown in normal condition), Ng (grown in 4% ai glyphosate), H (grown in 300mM NaCl) & Hg (grown in 300mM and 6%ai glyphosate). In vitro and in vivo studies of enzyme PAL, isolation, purification, substrate specificity, optimum pH and temperature, activators and inhibitors, kinetic properties, molecular weight by SDS-PAGE and the action of glyphosate on PAL enzyme was checked.

Keywords: Phenyl alanine ammonia lyase, Cajanus cajan, Rhizobium, SDS-PAGE, Glyphosate.