Estimation of Ofloxacin in Bulk and Formulation by Derivative UV-Spectrophotometric Methods

Jadhav SantoshA*, Shinde ShivajiA, Kharat RekhaA, Ansari AfaqueB, Tamboli AshpakC

ABSTRACT

Simple, fast and reliable spectrophotometric methods were developed for determination of Ofloxacin in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Methanol. The quantitative determination of the drug was carried out using the zero/0th, first, and second order method values measured at 298nm, 300nm and 300nm respectively. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Ofloxacin using 2-10μg/ml (r²=0.9938, r²=0.9992, r²=0.9945) for zero,first and second order spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Ofloxacin in pharmaceutical formulations.

Keywords: Ofloxacin, UV visible spectrophotometry, Zero, first and second order derivative spectrum