CHEMICAL COMPOSITION AND ANTIMICROBIAL EFFICACY OF CALCIUM HYDROXIDE WITH PEPPERMINT OIL AND TO COMPARE ITS EFFECT WITH CALCIUM HYDROXIDE WITH SALINE AGAINST ROOT CANAL PATHOGENS OF DECIDUOUS TEETH

Thosar Nilima1, Chandak Manoj 2, Bhat Manohar3, Basak Silpi4

1. Professor, Dept. of Pedodontics and Preventive Dentistry,
Sharad Pawar Dental College, Datta Meghe Institute of
Medical sciences (Deemed University), Wardha-442004,
Maharashtra, India.
2. Professor and Head, Dept. of Conservative Dentistry and
Endodontics, Sharad Pawar Dental College, Datta Meghe
Institute of Medical sciences (Deemed University),
Wardha-442004, Maharashtra, India.
3. Professor and Head, Dept. of Pedodontics & Preventive
Dentistry, Principal, Jaipur Dental College, Jaipur.
4. Professor, Dept. of Microbiology, Jawaharlal Nehru
Medical College, Datta Meghe Institute of Medical sciences
(Deemed University), Wardha-442004, Maharashtra, India.

ABSTRACT

Background: For endodontic treatment in deciduous teeth, thorough instrumentation and cleaning is required which may not be possible due to difficult anatomic configuration of deciduous teeth. So root canal filling material at least should possess antimicrobial efficacy. Therefore present study was planned to find out the bioactive ingredients and antimicrobial efficacy of calcium hydroxide mixed with peppermint oil (CaOH+P) and its effect was compared with routinely used calcium hydroxide with saline (CaOH+S) in the field of pediatric dentistry. Materials and methods: To find out the bioactive ingredients in peppermint oil, gas chromatography mass spectrophotometry was performed and for evaluating antimicrobial efficacy, agar diffusion method was employed in which, Muller Hincton agar was used. In MH agar plates, punching was done at two equidistant points and test materials were filled and kept in incubator at 37ºC for 24 hours. Zone of inhibition was measured in millimeter. Six times repetitions of the procedure was performed. Statistically data was analyzed by using ANOVA and Tukey’s post-hoc comparison test. P-value <0.05 was used for level of significance. Results: It was observed from the results that bioactive ingredients in peppermint oil were octanol (48.17%) followed by menthol (20.45%) and other ingredients were very less in quantity. Zone of inhibition obtained in CaOH+P oil paste against root canal pathogens in decreasing order were against Staph.aureus>E.coli>E.faecalis=P.aeruginosa with statistically significant difference (0.0001, p<0.05) while in CaOH+S paste, antimicrobial efficacy in decreasing order was E.Coli>Staph.aureus=E.faecalis>P.aeruginosa which was not significant statistically (0.373, p>0.05) Conclusion: Zone of inhibition obtained in CaOH+P oil paste for Staph.aureus were higher as compared to CaOH+S paste, while for other root canal pathogens i.e; E.coli, E.faecalis and P.aeruginosa, CaOH+S paste showed larger zones as compared to CaOH+P oil paste.

Keywords: Calcium hydroxide mixed with peppermint oil paste, calcium hydroxide mixed with saline paste, antimicrobial efficacy, gas chromatography mass spectrophotometry