NEW ANALYTICALMETHOD DEVELOPMENT AND VALIDATION OF ABACAVIR BY RP-HPLC

B.SRAVANTHI

OMEGA COLLEGE OF PHARMACY

ABSTRACT

A simple, precise, accurate, specific and RP-HPLC method was developed for determination of Abacavir in pharmaceutical formulation. BACKGROUND: The presented method is simple, since diluted samples are directly used without any preliminary chemical dramatization or purification steps. OBJECTIVE: The proposed method was validated for various ICH parameters like linearity, limit of detection, accuracy, precision, ruggedness, robustness, and system suitability. METHOD: A RP-HPLC assay utilized Symmetry C18 (4.6 x 150mm, 5 m, Make: XTerra) or equivalent with mobile phase composition of ph 7 buffer: acetonitrile [40:60] was used, and flow rate was 0.8 ml min-1 with UV detection at 285 nm. The retention time Abacavir of was 2.573 min respectively. The total RP-HPLC run time was 5 min. RESULT: Linearity was observed over concentration range of 20-60 μg/ml for Abacavir. Conclusion: Commercial tablet formulations and laboratory prepared dilutions were successfully analyzed using the developed methods.

Keywords: Abacavir, RP-HPLC, ph 7 buffer : acetonitrile [40:60].