Abdel Karim. M.1*, Sufian,A.2,Inas,O.3

DOI:

VOLUME-6, ISSUE-04

GC-MS Analysis and Antimicrobial Activity of Fixed Oil from Saudi Negella sativa (Ranunculaceae)

Abdel Karim. M.1*,

Sufian,A.2,

Inas,O.3

1- Sudan University of Science and Technology, Faculty of Science, Dept. of Chemistry

2- Taibah University , Faculty of Science, Dept. of Chemistry

3- University of Bahri, Faculty of Applied and Industrial Sciences

ABSTRACT

Constituents of Negella sativa seed oil were identified by GC-MS analysis. Major constituents are: methyl 12-hydroxy-9-octadecenoate(17.37%) , 9,12,15-octadecatrienoic acid (14.98%) , 9-octadecenoic acid methyl ester(12.84%), β-sitosterol (11.95%),9,12-octadecadienoic acid methyl ester(10.15%), 11-eicosenoic acid(9.67%), hexadecanoic acid(8.63%) , 13-docosenoic acid(5.20%) . The oil was extracted by two different methods (soxhlet and maceration) and each sample was screened separately for antimicrobial activity against a panel of pathogens.

Keywords: Negella sativa , Fixed oil, GC-MS, Antimicrobial activity.

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