TOBACCO PLANT (Nicotiana tabacum L) CRUDE EXTRACT AND ITS ANTIBACTERIAL ACTIVITY ON ESCHERICHIA coli AND STAPHYLOCOCCUS AUREUS

TYOKUSA A G*, ISHOMKEGH S A, ITOLO O.

Federal University of Agriculture, Makurdi, Nigeria

ABSTRACT

The Phytochemical constituents of aqueous and ethanolic crude extracts of dried and fresh Nicotiana tabacum leaves, stems, flowers and roots were examined and also investigated for antibacterial activity against Escherichia coli and Staphylococcus aureus. The Phytochemical constituents examined were flavonoids, alkaloids, cardiac glycosides, saponins, tannins, phenols, free anthracene, phytosterol, steroids and carbohydrates. Both the aqueous ethanolic extracts yielded phytochemical properties and also had antibacterial activity. The result also showed that all the four different parts of Nicotiana tabacum used had antibacterial activity with the leaves exhibiting highest activity. Generally, fresh Nicotiana tabacum extracts had higher antibacterial activity when compare to the dried parts of the plant. The Minimum Inhibitory Concentration (MIC) of both extracts against the test organisms was also determined

Keywords:

Phytochemical, crude extracts, Nicotiana tabacum, antibacterial and MIC