Loss of Protective Function of Paraoxonase Associated with Cardiovascular Diseases in Bangladeshi Origin

Md Amir Hossain, Israth Jahan Tuhin, Md. Mahfuzur Rahman, Muhammad Saiedullah

Manarat International University, Dhaka, Bangladesh

ABSTRACT

Cardiovascular diseases (CVDs) are the major health problem globally and considered to be the leading causes of death. The prevalence of CVDs is also rising among the adults in Bangladesh with the highest mortality rates. This study aimed to determine the serum Paraoxonase activity, lipid peroxidation product malon dialdehyde (MDA), and vitamin C status in subjects with cardiovascular diseases and to compare with apparently healthy subjects of Bangladeshi origin. In this case-control study, 34 male subjects from cardiac intervention and surgery (CIS) with an indication of angina, myocardial infarction, stroke and age, and body mass index-matched 24 apparently healthy males (controls) were included. Clinical and anthropometric data were recorded, and biochemical parameters were measured from serum samples. Serum Paraoxonase activity, MDA, and vitamin C concentrations were measured by standard spectrophotometric method. The mean age and body mass index (BMI) of the total subjects were 49±13 years and 22.6 ± 2.9 kg/m2, respectively. Serum Paraoxonase activity was significantly lower in subjects with CIS compared to control (113.7 ± 54 vs. 194.9±95 U/L, p=0.005). Serum MDA in CIS group was 7.7 ± 0.3 µmol/L and 6.6 ± 0.4 μmol/L in control group (p=0.0467). The mean values of serum vitamin C in CIS and control were 22.3 ± 4.4 and 24.8 ± 5.1 µmol/L, respectively. Vitamin C level was significantly lower in the CIS group compared to control (p = 0.057). Results of this study indicated that oxidative stress and loss of anti-atherogenic function of high-density lipoprotein particles Paraoxonase might be associated with cardiovascular diseases in subjects of Bangladeshi origin.

Keywords:

Oxidative stress, Paraoxonase, Cardiovascular disease