DOI:
VOLUME - 9 ISSUE - 1 MARCH - APRIL 2020
P. N. Presannakumaran, P. Jayasekhar
Pushpagiri College of Pharmacy, Medicity Campus, Tiruvalla, Kerala, India
ABSTRACT
The quantitative estimation of crude drugs present in an Ayurvedic formulation still remains a big challenge. A quantitative High Performance Thin Layer Chromatography (HPTLC) method was developed for the estimation of Udicya (Coleus vettiveroides), one of the herbal ingredients present in the Ayurvedic Polyherbal formulation ‘Patoladi Ghrita’. The formulation ‘Patoladi Ghrita’ is a medicated ghee originally described in the Ayurvedic book ‘Ashtangahridaya’ and is reproduced in the Ayurvedic Formulary of India, The methanol extracts of the crude drug Udicya and the formulation Patoladi Ghrita were run using a mobile phase Isopropyl alcohol : Water :: 9 : 1 (v/v) on a silica G 60 F254 stationary phase. Methanol extract of Udicya gave a spot with Rf value of 0.73. The methanol extract of Patoladi Ghrita also gave a spot with Rf value of 0.73. The chemical equivalency of these two spots was revealed by the same pattern of the UV spectrum. The estimation was done graphically by preparing Quarter Standard, Half Standard, Normal Standard, and Double Standard of the formulation. The methanol extract of the genuine drug and that of the four standards and four marketed samples were run using the same mobile phase. The reference spots on each track were scanned and integrated to get the AUC. A calibration plot was drawn between the concentration of standards and AUC of the reference spots. The concentration of Udicya in the samples was found from the corresponding AUC obtained for the reference peak of the samples. A regression coefficient of 0.951 was obtained. The developed HPTLC method is a simple and accurate one and can be used as a tool for the estimation of each ingredient in a Polyherbal formulation.
Keywords:
AUC, HPTLC, Coleus vettiveroides, Patoladi Ghrita, Regression Coefficient