Physio chemical characterization & antibacterial properties of biologically synthesized silver nanoparticles from aqueous extracts of ginger

Shraddha R. Yadav, Anahita V. Bhesania Hodiwala, Poonam Patil, Mansee Thakur*

MGM Medical College, MGMIHS, Navi Mumbai, India

ABSTRACT

In this work, the synthesis of stable silver nanoparticles (AgNPs)by aqueous extracts of ginger used as reducing and stabilizing agents, respectively and its antimicrobial activity against pathogenic bacteria causing urinary tract infection. After optimizing the synthesis parameters, samples were characterized by SEM, UV-Vis spectroscopy and FTIR. Biologically synthesized Ag-NPs was tested by two techniques i.e. Minimum inhibitory concentration(MIC) and Minimum bactericidal concentration(MBC) determination and agar well diffusion method to study the antibacterial properties. The initial syntheses of Ag nanoparticles were characterized by UV-Vis spectrophotometer and showed the surface Plasmon resonance band at 420nmsupported the reduction AgNO3 to AgNP. The average diameter of the prepared nanoparticles in solution was about 40-60 nm and were spherical in shape. Analysis of FTIR showed that secondary metabolites are responsible for bio-reduction in silver nanoparticles of silver nitrate. In this study, biologically synthesized Ag-NPs also exhibited strong antibacterial activity against bacteria (Escherichia coli) of the clinical isolates from patients suffering from urinary tract infection, shown effective inhibitory activity against sensitive strains of E. coli. (Susceptible to first-line antibiotics used to treat urinary tract infections. Results confirmed this protocol as simple, rapid, eco-friendly, non-toxic and an alternative to conventional physical/chemical method. It could be used as a safer alternative to antimicrobial and antibacterial agents for further research purposes.

Keywords:

Silver nanoparticles, Ginger, Antibacterial activity, UV–visible spectroscopy, SEM, FTIR, Urinary tract infection, E. coli