Interpretation of anti-urolithiatic activity of hibiscus rosa sinensis flower hydro alcoholic extract by uv spectrophotometry & turbidimetry

Manoj Kumar Sahu*, Dr. Gurdeep Singh

Oriental University, Indore, Madhya Pradesh, India

ABSTRACT

The main objective of this study is to determine efficacy of Hibiscus rosa sinensis flowers hydro alcoholic extract used for dissolve Calcium oxalate stone by treating stones with different concentrations of extracts. UV Spectroscopy instrument were used to detected absorbance of light by the crystal in the present in the herbal extract in different concentration. Turbidity meter is the instrument which is used to determine the turbidity in the sample. As a result, this study data show hydro alcoholic extract of the hibiscus rosa sinensis show significant reduction in crystal size reduction 10% to 60% inhibition of crystal formation in the presence of different concentration 100 μg, 500 μg ,1000 μg. The studies data support significant inhibition of crystal formation using hydro alcoholic extract of hibiscus flower for vitro anti-urolithic activity. The formation of suitable dosage form and use on the animal models will be increase the chance of better results.

Keywords:

Hibiscus Rosa sinensis, Calcium Oxalate, Nucleation, Aggregation assay, Crystallization inhibition rate