Development and in-vitro characterization of liposomal gel of bifonazole for topical use

Bhushan R. Rane, Aishwarya K Patil, Prashant L Pingale, Ashish S Jain, Dilip O Morani, Rajan V Kalamkar

Shri D D Vispute College of Pharmacy and Research Centre, Panvel, Maharashtra, India

ABSTRACT

The main objective of this study is to prepare and evaluate liposomes as a carrier for bifonazole. Liposomes are colloidal particles designed as concentric biomolecular layers that are capable of encapsulating drugs. Liposomes of soya lecithin and cholesterol were prepared in ten batches (L1- L10) with different ratio using thin film hydration technique. Prepared liposomes were evaluated for entrapment efficiency and particle size analysis. Liposomal gel formulations of prepared liposomes were formulated in six batches (L9F1- L9F6) and evaluated for drug content, in-vitro drug release study, particle size, zeta potential, polydispersity index (PDI) and stability study were performed. The entrapment efficiency, particle size, zeta potential and PDI of all liposome vesicle preparations were analyzed and it was found that the batch L9 liposome vesicles produced the entrapment efficiency 76.52 ± 0.175, particle size 119.1 nm, zeta potential -0.4, and PDI was found to be 0.406. Liposomal gel formulation was prepared. The drug content, in-vitro drug release study and stability study of all six batches of liposomal gel formulation were analyzed, and it was found that the L9F3 formulation shows better drug content, in-vitro drug release and stability at 5°C ± 2°C among all the formulations. Evaluation of prepared liposomal gel formulations was performed. After comparing all formulations of evaluation results on the basis of drug content and, in-vitro drug release and stability study, batch L9F3 formulation was considered as the best formulation.

Keywords:

Bifonazole, Antifungal, Liposomes, Soya lecithin, Cholesterol, Thin film hydration