Investigation of xanthine oxidase inhibitors in bioactive components of Jatropha podagrica stem bark

Truong Ngoc Minh, Yusuf Andriana, Bui Quang Minh, Nguyen Quang Trung, Chona de Guzman-Gelani

Department of Chemistry, College of Science and Mathematics, Mindanao State University, Iligan Institute of Technology, Iligan City, Philippines.

ABSTRACT

This study was conducted to examine the activity of antioxidants against xanthine oxidase in bioactive extracts from Jatropha podagrica stem bark. Among the extracts, the EtOAc extract showed the strongest antioxidant activity and xanthine oxidase inhibition. Column chromatography isolation using different ratios of ‘ethyl acetate-hexane’ (2:8, 3:7, and 4:6) yielded five fractions (M1-M5), which were identified and confirmed in the previous study. Specifically, the fractions included methyl gallate and gallic acid (M1); methyl gallate, gallic acid, and fraxetin (M2); methyl gallate, fraxetin and tomentin (M3); methyl gallate and fraxetin (M4); and fraxetin (M5). Employing allopurinol as the positive control (IC50= 20.2 μg/mL), M3, was found to have the highest level of xanthine oxidase inhibitors (IC50 = 69.7 μg/mL), followed by M5 (fraxetin, IC50 = 106.9 μg/mL). The results of this study proposed Jatropha podagrica as an alternative and potential source for the treatment of gout symptoms.

Keywords:

Xanthine Oxidase, Jatropha podagrica, Inhibitor, Stem, Bark.