ew analytical method development and validation of abacavir by RP-HPLC

B Sravanthi*

Omega college of pharmacy, Moradabad, Uttar Pradesh, India

Refer this article

B Sravanthi, 2017. New analytical method development and validation of abacavir by RP-HPLC. Journal of medical pharmaceutical and allied sciences, V 6 - I 2, Pages -154 – 158. Doi: https://doi.org/10.55522/jmpas.V6I2.0130.

ABSTRACT

A simple, precise, accurate, specific and RP-HPLC method was developed for determination of Abacavir in pharmaceutical formulation. The presented method is simple, since diluted samples are directly used without any preliminary chemical dramatization or purification steps. The proposed method was validated for various ICH parameters like linearity, limit of detection, accuracy, precision, ruggedness, robustness, and system suitability. A RP-HPLC assay utilized Symmetry C18 (4.6 x 150mm, 5 m, Make: XTerra) or equivalent with mobile phase composition of ph 7 buffer: acetonitrile was used, and flow rate was 0.8 ml min-1 with UV detection at 285 nm. The retention time Abacavir of was 2.573 min respectively. The total RP-HPLC run time was 5 min. Linearity was observed over concentration range of 20-60 µg/ml for Abacavir. Commercial tablet formulations and laboratory prepared dilutions were successfully analyzed using the developed methods.

Keywords:

Abacavir, RP-HPLC, ph 7 buffer: Acetonitrile.

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