Bioassay-guided fractionation and evaluation of anti-diabetic, anti-oxidant and anti-hyperlipidemic potential of punica granatum l. Var nana cultivated in egypt

Hanaa Hassan Eid, Kadriya Schafik E Deeb,Zeinab Youssef Ali, Ghada Farouk Metwally, Aliaa Moanes El-Fiki

Department of medicinal plants and natural products, Egyptian Drug Authority (EDA, formerly: NODCAR),Giza, Egypt.

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Hanaa Hassan Eid, Kadriya Schafik El Deeb,Zeinab Youssef Ali, Ghada Farouk Metwally, Aliaa Moanes El-Fiki, 2022. Bioassay-guided fractionation and evaluation of anti-diabetic, anti-oxidant and anti-hyperlipidemic potential of punica granatum l. Var nana cultivated in egypt. Journal of medical pharmaceutical and allied sciences, V 11 - I 6, Pages - 5440 – 5448. Doi: 10.55522/jmpas.V11I6.2491.

ABSTRACT

Punica granatum L. var. nana is an ornamental plant, yet it is underestimated medicinally compared to edible pomegranate. In this study, the ethanolic extract of the leaves(TEL)and its successive fractions were evaluated for anti-diabetic, anti-oxidant and anti-hyperlipidemic potentials in nicotinamide/streptozotocin(NA/STZ)-diabetic rats. Blood was measured for glucose, glycosylated haemoglobin(HbA1c%) and insulin levels.Anti-oxidant activity was assessed via measuring malondialdehyde(MDA), nitric oxide(NO), total anti-oxidant activity(TAC) and reduced glutathione(GSH). Anti-hyperlipidemic activity was evaluated via monitoring triglycerides(TG), total cholesterol(TC), low density lipoprotein-cholesterol(LDL-c), very low density lipoprotein-cholesterol(VLDL-c), high density lipoprotein-cholesterol(HDL-c) and atherogenic index(AI). Both TEL and ethyl acetate fraction(EAL) reduced glucose, HbA1c%, MDA, NO, TG, TC, LDL-c, VLDL-c and AI but increased plasma insulin, TAC, GSH and HDL-c. Therefore, the potent effect of TEL could be attributed to EAL, from whichseven phenoliccompounds were isolated.Nutritional composition was also investigated. Finally, leaves of the studied plant played a beneficial role in management of diabetes, controlling oxidative stress and improvement of lipid abnormalities.

Keywords:

Anti-diabetic, Anti-hyperlipidemic, Anti-oxidant, Bioactive metabolites, Nicotinamide -streptozotocin, Punica granatum L. var. nana

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