Study of phenolic content using LC–MS/MS technic in Saperavi grapevine shoots growing in Georgia

Natia Bokuchava, Tamaz Murtazashvili, Malkhaz Jokhadze, Koba Sivsivadze, Mariam Tatanashvili

Tbilisi State Medical University, Vazha Pshavela Ave, Tbilisi, Georgia

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Natia Bokuchava, Tamaz Murtazashvili, Malkhaz Jokhadze, Koba Sivsivadze, Mariam Tatanashvili, 2023. Study of phenolic content using LC–MS/MS technic in saperavi grapevine shoots growing in Georgia. Journal of medical pharmaceutical and allied sciences, V 12 - I 2, Pages - 5675 – 5679. Doi: 10.55522/jmpas.V12I2.4244.

ABSTRACT

Georgia is one of the world's oldest wine areas. Interest to by-products of vine processing is rising because they may be exploited as a source of bioactive compounds for pharmaceutical and cosmetic purposes, rather than being discarded. The aim of the present research was to study polyphenolic compounds content in Saperavi grapevine shoots extract. Shoots of Saperavi grapevine were collected in Kakheti, Georgia. Extract was preliminary prepared and concentrated 4-fold. Polyphenols were extracted using Methanol and total phenolic content (TPC) was measured with Folin-Ciocalteu (F-C) reagent in terms of Gallic acid. The qualitative composition and content of phenolic compounds was studied with Liquid Chromatography - tandem Mass Spectrometry (LC–MS/MS). The identification was achieved by comparison of the retention times (tR) and spectra characteristics of individual compounds with those in data library. As a result, 14 different phenolic compounds were isolated. Received results demonstrated possibility of using Georgian Saperavi grapevine shoots as a source of biologically active ingredients, for which studies should be proceeded accordingly. This research № PHDF-21-1607 has been supported by Shota Rustaveli National Science Foundation (SRNSFG).

Keywords:

Waste products, Polyphenols, Saperavi shoots, Folin-Ciocalteu, LC–MS/MS.

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