Chemical composition and antimicrobial efficacy of calcium hydroxide with peppermint oil and to compare its effect with calcium hydroxide with saline against root canal pathogens of deciduous teeth

Thosar Nilima, Chandak Manoj, Bhat Manohar, Basak Silpi*

Department of Pedodontics and Preventive Dentistry, Sharad Pawar Dental College, Datta Meghe Institute of Medical sciences (Deemed University), Maharashtra, India

Refer this article

Thosar Nilima, Chandak Manoj, Bhat Manohar, Basak Silpi, 2016. Chemical composition and antimicrobial efficacy of calcium hydroxide with peppermint oil and to compare its effect with calcium hydroxide with saline against root canal pathogens of deciduous teeth, V 5 - I 5, Pages -379 – 381. Doi: https://doi.org/10.55522/jmpas.V5I5.0099.

ABSTRACT

For endodontic treatment in deciduous teeth, thorough instrumentation and cleaning is required which may not be possible due to difficult anatomic configuration of deciduous teeth. So root canal filling material at least should possess antimicrobial efficacy. Therefore, present study was planned to find out the bioactive ingredients and antimicrobial efficacy of calcium hydroxide mixed with peppermint oil (CaOH+P) and its effect was compared with routinely used calcium hydroxide with saline (CaOH+S) in the field of pediatric dentistry. To find out the bioactive ingredients in peppermint oil, gas chromatography mass spectrophotometry was performed and for evaluating antimicrobial efficacy, agar diffusion method was employed in which, Muller Hincton agar was used. In MH agar plates, punching was done at two equidistant points and test materials were filled and kept in incubator at 37ºC for 24 hours. Zone of inhibition was measured in millimeter. Six times repetitions of the procedure were performed. Statistically data was analyzed by using ANOVA and Tukey’s post-hoc comparison test. P-value <0>E.coli>E.faecalis=P.aeruginosa with statistically significant difference (0.0001, p<0>Staph.aureus=E.faecalis>P.aeruginosa which was not significant statistically (0.373, p>0.05) Zone of inhibition obtained in CaOH+P oil paste for Staph.aureus were higher as compared to CaOH+S paste, while for other root canal pathogens i.e; E.coli, E.faecalis and P.aeruginosa, CaOH+S paste showed larger zones as compared to CaOH+P oil pastedisease

Keywords:

Calcium hydroxide mixed with peppermint oil paste, calcium hydroxide mixed with saline paste, antimicrobial efficacy, gas chromatography mass spectrophotometry.

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