DOI: https://doi.org/10.55522/jmpas.V15I2.7010
VOLUME 15 – ISSUE 2, MARCH - APRIL 2026
Chioma Akunnaya Ekenna-Ohanenye1, Uloaku Akubueze Nto-Ezimah2, Nto Johnson Nto*3, Faustina Chiamaka Irozulike1, Peace Kelechi Godson1
Department of Anatomy, Faculty of Basic Medical Sciences, College of Medicine, University of Nigeria, Enugu Campus, Nigeria
Refer this article
Chioma Akunnaya Ekenna-Ohanenye, Uloaku Akubueze Nto-Ezimah, Nto Johnson Nto, Faustina Chiamaka Irozulike, Peace Kelechi Godson, 2026. Sub-chronic effects of ethanolic leaf extract of Ocimum gratissimum on glucose homeostasis, haematological indices, oxidative stress markers, and liver histology in adult Wistar rats. Journal of medical pharmaceutical and allied sciences, V 15, I 2, Pages 35 – 48. Doi: https://doi.org/10.55522/jmpas.V15I2.7010
ABSTRACT
Ocimum gratissimum is widely used in traditional medicine for metabolic disorders; however, systematic evaluation of its metabolic, haematological, and hepatic safety profile remains limited. This study investigated the effects of ethanolic leaf extract of O. gratissimum on glucose homeostasis, haematological indices, oxidative stress markers, liver enzymes, and hepatic histology in rats. Sixteen adult Wistar rats were randomly assigned to four groups (n = 4). The control group received standard feed and water, while treatment groups were administered ethanolic leaf extract of O. gratissimum at doses of 100, 150, and 200 mg/kg body weight daily for 28 days. Blood glucose level was measured, alongside comprehensive haematological profiling. Hepatic oxidative stress markers, reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) were quantified. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) activities were assessed.
Keywords:
Ocimum gratissimum, Blood glucose, Oxidative stress, Liver enzymes, Haematology, Ethnopharmacology.