Development and validation of an eco-friendly UV spectrophotometric method using hydrotropic solubilizing agent for simultaneous estimation of esomeprazole and levosulpiride in solid dosage form

Pavan Kumar Patidar*, Mangal Singh Panwar

Gyanodaya Institute of Pharmacy, Neemuch, Madhya Pradesh, India

Refer this article

Pavan Kumar Patidar, Mangal Singh Panwar, 2026. Development and validation of an eco-friendly UV spectrophotometric method using hydrotropic solubilizing agent for simultaneous estimation of esomeprazole and levosulpiride in solid dosage form. Journal of medical pharmaceutical and allied sciences, V 15, I 4, Pages 29 – 31. Doi: https://doi.org/10.55522/jmpas.V15I4.7095.

ABSTRACT

The present study aimed to develop and validate a simple, economical, and eco-friendly UV spectrophotometric method for the simultaneous estimation of Esomeprazole (ESO) and Levosulpiride (LEV) in solid dosage forms using hydrotropic solubilization. Sodium benzoate (2 M) was selected as the hydrotropic agent to enhance the aqueous solubility of both drugs and eliminate the need for hazardous organic solvents. UV spectra were recorded in the range of 200–400 nm. The selected analytical wavelengths were 302 nm for Esomeprazole and 278 nm for Levosulpiride. Simultaneous equation and absorbance ratio methods were employed for quantitative analysis. The developed method was validated according to ICH Q2(R1) guidelines for linearity, accuracy, precision, specificity, robustness, LOD, and LOQ. The method demonstrated good linearity, accuracy, and precision with satisfactory recovery values. The proposed method offers a green analytical alternative for routine quality control analysis of Esomeprazole and Levosulpiride in pharmaceutical formulations.

Keywords:

Esomeprazole, Levosulpiride, Hydrotropy, Sodium benzoate, UV spectrophotometry, Green analytical chemistry.

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