Design and characterization of Gaur gum coated chitosan nanoparticles for delivery of 5-Fluorouracil for effective treatment of colon cancer

Garg Sweta1, Shukla Ajay1, Garg Ashish1*

Department of Pharmaceutics, Guru Ramdas Khalsa Institute of Science and Technology (pharmacy), Jabalpur, M.P. 483001

ABSTRACT

The present study was aimed to develop gaur gum (GG) coated chitosan nanoparticles as vector to provide successful delivery of 5-fluorouracil (5-FU). Guar gum anchored chitosan nanoparticles were prepared by cross linked with bifuctional PEG (NH2-PEG-COOH) for the treatment of colon cancer. Nanoparticle preparation was done by nanoprecipitation method to protect the drugs in gastric fluids and achieve a targeted drug delivery to colon. The characterization of GG coated chitosan nanoparticles was done by Fourier Transform Infrared Spectrophotometry (FT-IR), scanning electron microscopy (SEM), atomic force microscopy (AFM), average particle size in (Zeta seizer), Particle size analyzer. The drug polymer interactions were checked by Differential scanning calorimetry (DSC). The amount of 5-FU loading estimated by high performance liquid chromatography (HPLC) and in vitro drug release characterized. These studies revealed that controlled release behavior of Gaur gum coated chitosan NPs could be a potential alternative pharmaceutical formulation in treatment of colon cancer.

Keywords: Gaur gum, chitosan, nanoparticle, scanning electron microscopy (SEM), FT-IR, AFM.