Study of The soil DNA extraction and recovery procedures

Hamid kheyrodin

faculty of Semnan University, Semnan Province, Iran.

ABSTRACT

The focus of DNA extraction methods has moved to rapid performance of molecular techniques, avoiding extensive purification. Amplification of DNA from soil is often inhibited by co-purified contaminants. The need for identification of soil microbial community mainly depends on direct extraction of DNA from soil, a multifaceted environment that is a major pool for microbial genetic diversity. The soil DNA extraction procedures usually suffer from two major problems, namely, inappropriate rupturing of cells and contamination with humic substances. In the present study, five protocols for single type of rhizospheric soil were investigated and their comparison indicated that the inclusion of 120 mM phosphate buffered saline (PBS) for washing and mannitol in the lysis buffer allowed the processing of soil sample in minimal time with no specific equipment requirement. Furthermore, DNA purity and yield were also improved, which allowed the exploitation of genetic potential of soil microbes within soil sample thereby facilitating the amplification of metagenomic DNA. The effectiveness of methods was analyzed using random amplification of polymorphic DNA and the composition of indigenous microbial community depend on the DNA recovery method.

Keywords:

Soil, DNA, Microbial Diversity