A SYSTEMIC REVIEW ON NOVEL TECHNIQUES USED IN THE DETERMINATION OF NEUROPEPTIDE THROUGH THE ANALYTICAL TECHNIQUES

Nilesh C. Choudhary*, Dr. Karimunnisa Shaikh, Gholve Prachi, Karad Priyanka

PES’s Modern College of Pharmacy, Nigdi, Pune, Maharastra, India

ABSTRACT

Neuropeptides are a group of signaling molecules which are located in central nervous system. They control essential physiological function in animals like cardiovascular activity, energy, reproduction, growth, homeostasis, behavior, and stress response. Neuropeptides are the endogenous peptides they act as a neurotransmitter, neuromodulator and hormones. The detection process of neuropeptides is challenging due to their cleavage by peptidases and undergoes posttranslational modification. The in vivo isolation of neuropeptides occurs at very small concentrations as a significance of the efforts made to establish new neuropeptide detection techniques. Here, we examine these view points and the related strategies, and new techniques concentrating on advancements that have exhibited potential in propelling the field lately.

Keywords:

Neuropeptides, MALDI MS, Nuclear magnetic resonance, Radioimmunoassay, Ascorbic acid, In Situ Hybridization