Development and validation of methodology to study the genetic polymorphism of organic cation transporter 1 (OCT1) - rs622342: A Prospective open-label study

Swathi Swaroopa Borra, Diya Choorapra, Gopika Nair Hemachandran Chithra, Jegathis Kumar Mani, Sadagoban Gopal Krishnamoorthy, Arun Kanniyappan Parthasarathy*

Department of Pharmacy Practice, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamil Nadu, India

ABSTRACT

The main objective is to develop and validate the methodology to study the genetic polymorphism of Organic cation transporter (OCT1) rs622342. The Reverse Transcriptase-PCR method for identifying the OCT1 rs622342 genetic polymorphism was developed and validated. This study is a prospective open-label study conducted over a period of six months with 61 South Indian healthy male volunteers. Phenol chloroform method was used for the DNA isolation, which was subjected to PCR for identifying the polymorphism. The method was successfully developed. After the addition of restriction enzyme (PsiI) in the ten samples, none of the samples showed fragmentation, which shows that there is a lack of rs622342 genetic polymorphism in the selected ten samples.

Keywords:

Genetic polymorphism, Organic cation transporter, Methodology & Validation, Healthy volunteers