Immunohistochemical expression of epidermal growth factor receptor (EGFR) and p53 in Gliomas and correlation with clinicopathological parameters

Prajna Das, Debahuti Mohapatra, Sushree Sumedha Dash, Pranita Mohanty

Department of Pathology, IMS and Sum Hospital, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, India

ABSTRACT

The most common primary central nervous system (CNS) tumor originating from glial cells is Glioma. It accounts for approximately 80% of all primary malignant brain tumors. Our aim was to study the expressionof Epidermal Growth Factor Receptors (EGFR) and p53 by Immunohistochemistry (IHC) in Gliomas and to correlate with various histological types and grades as well as clinical parameters like age and gender of patients. It was an ambispective study for 1year and 2 months conducted in IMS Sum Hospital, Bhubaneswar. 38 cases of Gliomas operated in Neurosurgery Department were studied. Histological typing & grading were done according to WHO 2016 classification of CNS tumors. EGFR & p53 study was done by immunohistochemistry (IHC).Out of 38 cases, 14 cases (36.8%), 6 cases (15.7%), 6 cases (15.7%), 4 cases (10.5%), 6 cases (15.7%) and 2 cases (5.2%) were Astrocytoma, Oligodendroglioma, Glioblastoma, GBM with oligodendroglioma, Gliosarcoma and Ependymoma respectively. 2 cases (5.2%), 14 cases (36.8%), 6 cases (15.7%) and 16 cases (42.1%) were of Grade- I, II, III and IV respectively. In Grade I tumors, there was negative expression of both EGFR and p53. EGFR and p53 was positive in 57.14%, 71.42�ses of Grade II tumors respectively. Both the markers were 100% positive in Grade III and Grade IV tumors. Thus, there was positive and negative correlation with expression of both EGFR & p53 in higher grades low grades of tumors.

Keywords:

EGFR, Glioblastoma (GBM), p53, Immunohistochemical analysis, Clinical parameters.